Anticorps Recombinant de lapin anti-DDX24

DDX24 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241145G10

N° de cat : 84103-6-RR

Synonymes

EC:3.6.4.13, DDX 24, ATP-dependent RNA helicase DDX24, 241145G10

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Galerie de données de validation

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  WB analysis using 84103-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84103-6-RR (DDX24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84103-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX24 antibody (84103-6-RR, Clone: 241145G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U-2 OS using 84103-6-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug DDX24 Recombinant antibody (84103-6-RR, Clone:241145G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84103-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84103-6-RR against Human DDX24 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules A549, cellules COLO 320, cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): U-2 OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84103-6-RR cible DDX24 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DDX24 Protéine recombinante Ag8407
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC008847
• Symbole du gène: DDX24
• Identification du gène (NCBI): 57062
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX24 is a family member of Asp-Glu-Ala-Asp (DEAD) box containing RNA helicases, and DEAD-box RNA helicases are characterized by a conserved DEAD motif. DDX24 was associated with cancer development, viral infection,and vascular malformation.

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