Anticorps Monoclonal anti-DDX3

DDX3 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D3A1

N° de cat : 67915-1-PBS

Synonymes

DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X

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Galerie de données de validation

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  WB analysis using 67915-1-Ig (same clone as 67915-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67915-1-Ig (DDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67915-1-Ig (same clone as 67915-1-PBS)

  WB result of DDX3 antibody (67915-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells. This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67915-1-Ig (same clone as 67915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67915-1-Ig (DDX3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67915-1-Ig (same clone as 67915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67915-1-Ig (DDX3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 67915-1-Ig (same clone as 67915-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human DDX3 (67915-1-Ig, Clone:1D3A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67915-1-PBS cible DDX3 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DDX3 Protéine recombinante Ag29972
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 71-73 kDa
• Numéro d’acquisition GenBank: BC011819
• Symbole du gène: DDX3
• Identification du gène (NCBI): 1654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DDX3X, also named DBX, DDX3, and HLP2, belongs to the DEAD-box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73kd is the target band. 60-65kd is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

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