Anticorps Recombinant de lapin anti-DDX3

• Phare
• Validé par KD/KO

DDX3 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5C12

N° de cat : 81903-1-PBS

Synonymes

DDX3X, 5C12, ATP-dependent RNA helicase DDX3X, CAP-Rf, DBX

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Galerie de données de validation

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  WB analysis using 81903-1-RR (same clone as 81903-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81903-1-RR (DDX3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

  WB result of DDX3 antibody (81903-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells. This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (81903-1-RR, Clone: 5C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DDX3 Recombinant antibody (81903-1-RR, Clone:5C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

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Informations sur le produit

81903-1-PBS cible DDX3 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DDX3 Protéine recombinante Ag1614
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC011819
• Symbole du gène: DDX3
• Identification du gène (NCBI): 1654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

{{ptg:RelatedPrimaryAntibodies}}