Anticorps Polyclonal de lapin anti-DDX3Y

DDX3Y Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14041-1-PBS

Synonymes

ATP-dependent RNA helicase DDX3Y, DBY, DEAD box protein 3, Y-chromosomal, EC:3.6.4.13

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Galerie de données de validation

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  WB analysis using 14041-1-AP (same clone as 14041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14041-1-AP (DDX3Y antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14041-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 14041-1-AP (same clone as 14041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14041-1-AP (DDX3Y antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14041-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 14041-1-AP (same clone as 14041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 14041-1-AP (DDX3Y antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 14041-1-AP (same clone as 14041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 14041-1-AP (DDX3Y antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 14041-1-AP (same clone as 14041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14041-1-AP (DDX3Y antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14041-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14041-1-AP (same clone as 14041-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DDX3Y antibody (14041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14041-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14041-1-PBS cible DDX3Y dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX3Y Protéine recombinante Ag5110
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC034942
• Symbole du gène: DDX3Y
• Identification du gène (NCBI): 8653
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. DDX3Y is one member of DEAD box RNA helicases, encode by the gene locate on the chromosome Y in the azoospermia factor A region. Polyclonal anti-DDX3Y antibodies (14041-1-AP) are produced by immunizing animals with C-terminus of DDX3Y and detect an 73-kDa band. As DDX3Y has a structural homolog with DDX3X, the antibody can recognize both.

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