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Anticorps Polyclonal de lapin anti-DDX4,VASA

DDX4,VASA Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

ELISA

Conjugaison

Non conjugué

N° de cat : 12888-1-AP

Synonymes

DDX4, DEAD box protein 4, VASA, Vasa homolog

Galerie de données de validation

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX4/VASA Polyclonal antibody (12888-1-AP)

    IHC staining of mouse testis using 12888-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12888-1-AP (DDX4/VASA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX4/VASA Polyclonal antibody (12888-1-AP)

    IHC staining of mouse testis using 12888-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12888-1-AP (DDX4/VASA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Applications testées

Dilution recommandée

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

12888-1-AP cible DDX4,VASA dans les applications de ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène DDX4,VASA Protéine recombinante Ag3919
Nom complet DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Masse moléculaire calculée690aa,76 kDa; 724aa,79 kDa
Numéro d’acquisition GenBankBC047455
Symbole du gène DDX4
Identification du gène (NCBI) 54514
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].