Anticorps Monoclonal anti-DDX4,VASA

DDX4,VASA Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1F12C3

N° de cat : 67147-2-PBS

Synonymes

DDX4, 1F12C3, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX4, VASA

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Galerie de données de validation

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  WB analysis of SKOV-3 using 67147-2-Ig (same clone as 67147-2-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 67147-2-Ig (same clone as 67147-2-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

• 

  WB analysis of JAR using 67147-2-Ig (same clone as 67147-2-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 67147-2-Ig (same clone as 67147-2-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

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Informations sur le produit

67147-2-PBS cible DDX4,VASA dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DDX4,VASA Protéine recombinante Ag11630
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• Masse moléculaire calculée: 690aa,76 kDa; 724aa,79 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC047455
• Symbole du gène: DDX4
• Identification du gène (NCBI): 54514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

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