Anticorps Polyclonal de lapin anti-DDX42

DDX42 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31211-1-PBS

Synonymes

RNA helicase related protein, RNA helicase like protein, RHELP, EC:3.6.4.13, DEAD box protein 42

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Galerie de données de validation

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  WB analysis using 31211-1-AP (same clone as 31211-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31211-1-AP (DDX42 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31211-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 31211-1-AP (same clone as 31211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31211-1-AP (DDX42 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31211-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 31211-1-AP (same clone as 31211-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX42 antibody (31211-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31211-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31211-1-PBS cible DDX42 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX42 Protéine recombinante Ag34762
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC015505
• Symbole du gène: DDX42
• Identification du gène (NCBI): 11325
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DDX42 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 42) is a member of the DEAD box RNA helicase superfamily. A C-terminal portion of DDX42 interacts with the pro-apoptotic factor, apoptosis-stimulating protein of p53 protein 2 (ASPP2), thereby inhibiting its action (PMID: 30834153).

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