Anticorps Recombinant de lapin anti-DDX47

DDX47 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240193C4

N° de cat : 83287-3-PBS

Synonymes

MSTP162, HQ0256, EC:3.6.4.13, E4 DBP, DEAD box protein 47

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Galerie de données de validation

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  WB analysis using 83287-3-RR (same clone as 83287-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83287-3-RR (DDX47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX47 Recombinant antibody (83287-3-RR, Clone:240193C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

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Informations sur le produit

83287-3-PBS cible DDX47 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DDX47 Protéine recombinante Ag11953
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
• Masse moléculaire calculée: 455 aa, 51 kDa
• Numéro d’acquisition GenBank: BC068009
• Symbole du gène: DDX47
• Identification du gène (NCBI): 51202
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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