Anticorps Polyclonal de lapin anti-DDX5

• Phare
• Validé par KD/KO

DDX5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10804-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 10804-1-AP (same clone as 10804-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10804-1-AP (same clone as 10804-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5, antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10804-1-AP (same clone as 10804-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10804-1-AP (same clone as 10804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10804-1-AP (DDX5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10804-1-AP (same clone as 10804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10804-1-AP (DDX5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10804-1-PBS cible DDX5 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX5 Protéine recombinante Ag1237
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 65-68 kDa
• Numéro d’acquisition GenBank: BC016027
• Symbole du gène: DDX5
• Identification du gène (NCBI): 1655
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation. For the reason that DDX5 can be phosphorylated and dephosphorylated at several sites, at some cases the molecular weight of DDX5 is less than 68kD, approximately 65kd

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