Anticorps Monoclonal anti-DDX5

DDX5 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

2D3D2

Avis (1)

N° de cat : 67025-1-Ig

Synonymes

2D3D2, DDX5,p68, EC:3.6.4.13, G17P1, HELR

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Galerie de données de validation

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  WB analysis using 67025-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 67025-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67025-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 67025-1-Ig

  IP result of anti-DDX5 (IP:67025-1-Ig, 5ug; Detection:67025-1-Ig 1:1000) with HeLa cells lysate 1600 ug.

• 

  IHC staining of human colon cancer using 67025-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67025-1-Ig (DDX5 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67025-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67025-1-Ig (DDX5 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67025-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (67025-1-Ig, Clone: 2D3D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 67025-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (67025-1-Ig, Clone: 2D3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules NCI-H1299, cellule HEK-293, cellules 4T1, cellules A431, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules HT-29, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

67025-1-Ig cible DDX5 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DDX5 Protéine recombinante Ag24821
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: NM_004396
• Symbole du gène: DDX5
• Identification du gène (NCBI): 1655
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation.

Publications

Species	Application	Title
human	CoIP	Theranostics; LncRNA PRADX-mediated recruitment of PRC2/DDX5 complex suppresses UBXN1 expression and activates NF-κB activity, promoting tumorigenesis.; Authors - Yansheng Li
human	WB,IP	Cancer Biol Ther; VAMP8 suppresses the metastasis via DDX5/β-catenin signal pathway in osteosarcoma; Authors - Shuo Yang
mouse,human,bovine	WB,IF,CoIP	bioRxiv; Maf-family bZIP transcription factor NRL interacts with RNA-binding proteins and R-loops in retinal photoreceptors; Authors - Ximena Corso-Díaz
human	WB	Nucleic Acids Res; N 2-Alkyl-dG lesions elicit R-loop accumulation in the genome; Authors - Yinan Wang
human	WB	Commun Biol; Hypoxia-induced circPLOD2a/b promotes the aggressiveness of glioblastoma by suppressing XIRP1 through binding to HuR; Authors - Aixin Yu
human	WB,IF	Elife; Maf-family bZIP transcription factor NRL interacts with RNA-binding proteins and R-loops in retinal photoreceptors; Authors - Ximena Corso Diaz

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Specific band detected for DDX5 from HeLa cells extract (1/1000 dilution, Overnight incubation at 4°C).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

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