Anticorps Polyclonal de lapin anti-DDX51

DDX51 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20149-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 20149-1-AP (same clone as 20149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 20149-1-AP (same clone as 20149-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 20149-1-AP (same clone as 20149-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 20149-1-AP (same clone as 20149-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 20149-1-AP (same clone as 20149-1-PBS)

  IP result of anti-DDX51 (IP:20149-1-AP, 4ug; Detection:20149-1-AP 1:1000) with K-562 cells lysate 1820 ug. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20149-1-PBS cible DDX51 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX51 Protéine recombinante Ag13691
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 51
• Masse moléculaire calculée: 666 aa, 72 kDa
• Poids moléculaire observé: 72-80 kDa
• Numéro d’acquisition GenBank: BC040185
• Symbole du gène: DDX51
• Identification du gène (NCBI): 317781
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The DEAD-box family (DDX) consists of a group of RNA helicases with the activity to hydrolyze ATP and to unwind RNA. The major physiological role of this family was to regulate RNA metabolism including ribosome assembly, RNA splicing, and pre-rRNA processing. DDX51 was a new gene in DDX family composed of 666 amino acids. DDX51 gene was a member of RNA helicases family in charge of regulating RNA metabolism(PMID: 31966432).

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