Anticorps Polyclonal de lapin anti-RIG-1/DDX58

• Phare
• Validé par KD/KO

RIG-1/DDX58 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

41

N° de cat : 20566-1-AP

Synonymes

DDX58, DEAD box protein 58, RIG 1, RIG I, RIG-I

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Galerie de données de validation

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  WB analysis using 20566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20566-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20566-1-AP

  WB result of RIG-1/DDX58 antibody (20566-1-AP; 1:2000; room temperature for 1.5 hours) with wild-type and RIG-1/DDX58 knockout A431 cells.

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  WB analysis of MCF-7 using 20566-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20566-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20566-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 20566-1-AP

  IP Result of anti-DDX58 (IP:20566-1-AP, 4ug; Detection:20566-1-AP 1:300) with HepG2 cells lysate 3600ug.

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  IF Staining of HepG2 using 20566-1-AP

  Immunofluorescent analysis of HepG2 cells, using DDX58 antibody 20566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of H9C2 using 20566-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using RIG-1/DDX58 antibody (20566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431, cellules HepG2, cellules MCF-7, cellules MOLT-4, tissu cérébral de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IF: cellules HepG2, cellules H9C2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 37 publications below
• IHC: See 6 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

20566-1-AP cible RIG-1/DDX58 dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• Masse moléculaire calculée: 107 kDa
• Poids moléculaire observé: 101/106 kDa
• Numéro d’acquisition GenBank: NM_014314
• Symbole du gène: DDX58
• Identification du gène (NCBI): 23586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human	WB	Nat Microbiol; IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.; Authors - Zhimin Jiang
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	WB	EBioMedicine; An HBV susceptibility variant of KNG1 modulates the therapeutic effects of interferons α and λ1 in HBV infection by promoting MAVS lysosomal degradation; Authors - Bin Zhang
pig	WB	Autophagy; STING1 is essential for an RNA-virus triggered autophagy.; Authors - Rui Zhang ; KD Validated
human	WB	Elife; Ribosome biogenesis restricts innate immune responses to virus infection and DNA.; Authors - Christopher Bianco