Anticorps Monoclonal anti-RIG-1/DDX58

RIG-1/DDX58 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

3B10F5

N° de cat : 67556-1-Ig

Synonymes

DDX58, RIG-I, 3B10F5, Antiviral innate immune response receptor RIG-I, ATP-dependent RNA helicase DDX58

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Galerie de données de validation

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  WB analysis using 67556-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67556-1-Ig (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67556-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RIG-1/DDX58 antibody (67556-1-Ig, Clone: 3B10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A-253, cellules HeLa, cellules MDA-MB-231, cellules SW-1990
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

67556-1-Ig cible RIG-1/DDX58 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, duck
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RIG-1/DDX58 Protéine recombinante Ag18591
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• Masse moléculaire calculée: 925 aa, 106 kDa
• Poids moléculaire observé: 101/106 kDa
• Numéro d’acquisition GenBank: BC132786
• Symbole du gène: RIG-1/DDX58
• Identification du gène (NCBI): 23586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58,which has two isoform with the MW of 106 and 101 kDa.

Publications

Species	Application	Title
human	IF	Autophagy; N protein of PEDV plays chess game with host proteins by selective autophagy; Authors - Xueying Zhai
		J Virol; RBM14 inhibits the replication of porcine epidemic diarrhea virus by recruiting p62 to degrade nucleocapsid protein through the activation of autophagy and interferon pathway; Authors - Xiaoquan Wang
human	WB	Nat Commun; TRIM25 predominately associates with anti-viral stress granules; Authors - Zehua Shang
human	WB	Redox Biol; Nlrp6 protects from corticosterone-induced NSPC ferroptosis by modulating RIG-1/MAVS-mediated mitophagy; Authors - Jingyan Shen
duck	WB	Poult Sci; Matrine relieved DHAV-1-induced hepatocyte excessive interferon and pyroptosis by activating mitophagy; Authors - Weiran Wang
		Vet Microbiol; BTG3 inhibits porcine epidemic diarrhea virus replication by promoting viral S2 protein degradation through the autophagy and proteasome pathways; Authors - Qingxiao Zhu

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