Anticorps Polyclonal de lapin anti-DDX59

DDX59 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 32948-1-PBS

Synonymes

DEAD box protein 59, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX59, Zinc finger HIT domain-containing protein 5, ZNHIT5

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Galerie de données de validation

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  WB analysis using 32948-1-AP (same clone as 32948-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32948-1-AP (DDX59 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32948-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 32948-1-AP (same clone as 32948-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 32948-1-AP (DDX59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32948-1-PBS in a different storage buffer formulation.

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Informations sur le produit

32948-1-PBS cible DDX59 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX59 Protéine recombinante Ag37657
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 59
• Masse moléculaire calculée: 619 aa, 69 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC041801
• Symbole du gène: DDX59
• Identification du gène (NCBI): 83479
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DEAD box proteins are multifunctional proteins involved in every aspect in RNA metabolism and have essential roles in many cellular activities. DDX59 is overexpressed in lung adenocarcinoma. DDX59 knockdown reduced cell proliferation, anchorage-independent cell growth, and caused reduction of tumor formation in immunocompromised mice. In multiple lung cancer cells, DDX59 knockdown inhibits DNA synthesis; wild-type DDX59 but not helicase-defective mutant of DDX59 enhances DNA synthesis. DDX59 knockdown caused reduction of MCM protein levels, decreased the loading of MCM ring protein onto chromatin, and therefore inhibited DNA replication. DDX59 has an important role in lung cancer development through promoting DNA replication (PMID: 28090355).

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