Anticorps Polyclonal de lapin anti-DEAF1

DEAF1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 18323-1-AP

Synonymes

DEAF1, NUDR, SPN, Suppressin, ZMYND5

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Galerie de données de validation

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  WB analysis using 18323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of Jurkat using 18323-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DEAF1 antibody (18323-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules BxPC-3, cellules Jurkat, cellules SH-SY5Y
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral humain, tissu cutané humain, tissu de cancer du foie humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18323-1-AP cible DEAF1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DEAF1 Protéine recombinante Ag13173
• Nom complet: deformed epidermal autoregulatory factor 1 (Drosophila)
• Masse moléculaire calculée: 565 aa, 59 kDa
• Poids moléculaire observé: 60-63 kDa, 72 kDa
• Numéro d’acquisition GenBank: BC053322
• Symbole du gène: DEAF1
• Identification du gène (NCBI): 10522
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.