Anticorps Monoclonal anti-DEK

• Phare
• Validé par KD/KO

DEK Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

5E3A6

N° de cat : 66194-1-PBS

Synonymes

5E3A6, D6S231E

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Galerie de données de validation

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  WB analysis using 66194-1-Ig (same clone as 66194-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66194-1-Ig (DEK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66194-1-Ig (same clone as 66194-1-PBS)

  WB result of DEK antibody (66194-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEK transfected HEK-293 cells. This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66194-1-Ig (DEK antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66194-1-Ig (DEK antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66194-1-Ig (same clone as 66194-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DEK antibody (66194-1-Ig, Clone: 5E3A6 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66194-1-PBS cible DEK dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DEK Protéine recombinante Ag8225
• Nom complet: DEK oncogene
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC035259
• Symbole du gène: DEK
• Identification du gène (NCBI): 7913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

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