Anticorps Polyclonal de lapin anti-DENND2D

DENND2D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 33113-1-PBS

Synonymes

DENN Domain Containing 2D , DENN domain-containing protein 2D

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Galerie de données de validation

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  WB analysis using 33113-1-AP (same clone as 33113-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33113-1-AP (DENND2D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 33113-1-AP (same clone as 33113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 33113-1-AP (same clone as 33113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 33113-1-AP (same clone as 33113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 33113-1-AP (same clone as 33113-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DENND2D antibody (33113-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

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Informations sur le produit

33113-1-PBS cible DENND2D dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DENND2D Protéine recombinante Ag36381
• Nom complet: DENN/MADD domain containing 2D
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC004557
• Symbole du gène: DENND2D
• Identification du gène (NCBI): 79961
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DENND2D (DENN domain-containing protein 2D) is a protein containing a DENN domain and belongs to the family of membrane transport proteins, participating in intracellular membrane transport and signal transduction processes. The DENND2D protein comprises several conserved domains, such as the uDENN, DENN, and dDENN domains, which are widely present in various signaling proteins. In cancer research, the expression and methylation status of DENND2D hold significant prognostic value. DENND2D may serve as a novel tumor biomarker for predicting cancer progression and recurrence.

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