Anticorps Polyclonal de lapin anti-DEPDC6

DEPDC6 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21312-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 21312-1-AP (same clone as 21312-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 21312-1-AP (same clone as 21312-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 21312-1-AP (same clone as 21312-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 21312-1-AP (same clone as 21312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 21312-1-AP (same clone as 21312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21312-1-PBS cible DEPDC6 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DEPDC6 Protéine recombinante Ag15959
• Nom complet: DEP domain containing 6
• Masse moléculaire calculée: 409 aa, 46 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC024746
• Symbole du gène: DEPDC6
• Identification du gène (NCBI): 64798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.

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