Anticorps Monoclonal anti-DFFA/DFF45

DFFA/DFF45 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F6H9

N° de cat : 68177-1-PBS

Synonymes

DFFA,DFF45, DFF45, DFFA, 1F6H9, DFF alpha

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Galerie de données de validation

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  WB analysis using 68177-1-Ig (same clone as 68177-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68177-1-Ig (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DFFA,DFF45 antibody (68177-1-Ig, Clone: 1F6H9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug DFFA,DFF45 Monoclonal antibody (68177-1-Ig, Clone:1F6H9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50354-1

  Cytometric bead array standard curve of MP50354-1, DFFA,DFF45 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68177-1-PBS. Detection antibody: 68177-2-PBS. Standard:Ag32657. Range: 3.125-200 ng/mL.

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  Cytometric bead array standard curve of MP50354-2

  Cytometric bead array standard curve of MP50354-2, DFFA,DFF45 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68177-3-PBS. Detection antibody: 68177-1-PBS. Standard:Ag32657. Range: 6.25-200 ng/mL.

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Informations sur le produit

68177-1-PBS cible DFFA/DFF45 dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DFFA/DFF45 Protéine recombinante Ag32657
• Nom complet: DNA fragmentation factor, 45kDa, alpha polypeptide
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC000037
• Symbole du gène: DFFA
• Identification du gène (NCBI): 1676
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.

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