Anticorps Recombinant de lapin anti-DFFA,DFF45

DFFA,DFF45 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242915D6

N° de cat : 85220-5-RR

Synonymes

DFF45, DFFA, 242915D6, DFF alpha, DFF1

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Galerie de données de validation

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  WB analysis using 85220-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85220-5-RR (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85220-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DFFA,DFF45 antibody (85220-5-RR, Clone: 242915D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85220-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85220-5-RR against Human DFFA,DFF45 were performed. The affinity constant is 1.47 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules C6, cellules HEK-293, cellules K-562, cellules MCF-7, tissu cérébral de rat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85220-5-RR cible DFFA,DFF45 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DFFA,DFF45 Protéine recombinante Ag0244
• Nom complet: DNA fragmentation factor, 45kDa, alpha polypeptide
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC000037
• Symbole du gène: DFFA
• Identification du gène (NCBI): 1676
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

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