Anticorps Recombinant de lapin anti-DFNA5/GSDME

DFNA5/GSDME Recombinant Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

240394D2

N° de cat : 83454-6-PBS

Synonymes

DFNA5, Gasdermin E, Gasdermin-E, GSDME, 240394D2

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Galerie de données de validation

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  WB analysis using 83454-6-RR (same clone as 83454-6-PBS)

  Untreated and etoposide-treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 83454-6-RR (DFNA5/GSDME antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83454-6-PBS in a different storage buffer formulation.

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  WB analysis using 83454-6-RR (same clone as 83454-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83454-6-RR (DFNA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83454-6-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 83454-6-RR (same clone as 83454-6-PBS)

  IP result of anti-DFNA5/GSDME (IP:83454-6-RR, 4ug; Detection:83454-6-RR 1:1000) with SH-SY5Y cells lysate 1840 ug. This data was developed using the same antibody clone with 83454-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83454-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83454-6-RR against Human DFNA5 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83454-6-PBS cible DFNA5/GSDME dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DFNA5/GSDME Protéine recombinante Ag3746
• Nom complet: deafness, autosomal dominant 5
• Masse moléculaire calculée: 496 aa, 55 kDa
• Poids moléculaire observé: 55 kDa, 35 kDa
• Numéro d’acquisition GenBank: BC019689
• Symbole du gène: DFNA5
• Identification du gène (NCBI): 1687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

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