Anticorps Recombinant de lapin anti-DFNA5/GSDME

DFNA5/GSDME Recombinant Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

240394D2

N° de cat : 83454-6-RR

Synonymes

DFNA5, Gasdermin E, Gasdermin-E, GSDME, 240394D2

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Galerie de données de validation

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  WB analysis using 83454-6-RR

  Untreated and etoposide-treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 83454-6-RR (DFNA5/GSDME antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83454-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83454-6-RR (DFNA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 83454-6-RR

  IP result of anti-DFNA5/GSDME (IP:83454-6-RR, 4ug; Detection:83454-6-RR 1:1000) with SH-SY5Y cells lysate 1840 ug.

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  Affinity and Kinetic Characterization of 83454-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83454-6-RR against Human DFNA5 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83454-6-RR cible DFNA5/GSDME dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DFNA5/GSDME Protéine recombinante Ag3746
• Nom complet: deafness, autosomal dominant 5
• Masse moléculaire calculée: 496 aa, 55 kDa
• Poids moléculaire observé: 55 kDa, 35 kDa
• Numéro d’acquisition GenBank: BC019689
• Symbole du gène: DFNA5
• Identification du gène (NCBI): 1687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

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