Anticorps Polyclonal de lapin anti-DFNA5/GSDME

DFNA5/GSDME Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 31363-1-AP

Synonymes

DFNA5, Gasdermin E, Gasdermin-E, GSDME, deafness, autosomal dominant 5

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Galerie de données de validation

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  WB analysis using 31363-1-AP

  Untreated, and etoposide (60uM, 14h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 31363-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 31363-1-AP

  IP result of anti-DFNA5/GSDME (IP:31363-1-AP, 4ug; Detection:31363-1-AP 1:800) with SH-SY5Y cells lysate 6790 ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 31363-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DFNA5 antibody (31363-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules A549, tissu cérébral de souris
• Résultats positifs en IP: cellules SH-SY5Y,
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31363-1-AP cible DFNA5/GSDME dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DFNA5/GSDME Protéine recombinante Ag35186
• Nom complet: deafness, autosomal dominant 5
• Masse moléculaire calculée: 496 aa, 55 kDa
• Poids moléculaire observé: 55 kDa, 35 kDa, 25 kDa
• Numéro d’acquisition GenBank: BC019689
• Symbole du gène: DFNA5
• Identification du gène (NCBI): 1687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

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