Anticorps Monoclonal anti-DFNA5/GSDME

DFNA5/GSDME Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D9C3

N° de cat : 67731-1-PBS

Synonymes

DFNA5, Gasdermin E, Gasdermin-E, GSDME, 1D9C3

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Galerie de données de validation

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  WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

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  WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 67731-1-Ig (same clone as 67731-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human DFNA5/GSDME (67731-1-Ig, Clone:1D9C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67731-1-PBS cible DFNA5/GSDME dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DFNA5/GSDME Protéine recombinante Ag30514
• Nom complet: deafness, autosomal dominant 5
• Masse moléculaire calculée: 496 aa, 55 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC019689
• Symbole du gène: DFNA5
• Identification du gène (NCBI): 1687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.

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