Anticorps Polyclonal de lapin anti-DGAT1

DGAT1 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(16)

Avis (5)

N° de cat : 11561-1-AP

Synonymes

ACAT related gene product 1, AGRP1, ARGP1, DGAT, DGAT1, Diglyceride acyltransferase

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Galerie de données de validation

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  IHC staining of human hepatocirrhosis using 11561-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11561-1-AP (DGAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 11561-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11561-1-AP (DGAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu de cirrhose hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

11561-1-AP cible DGAT1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DGAT1 Protéine recombinante Ag2123
• Nom complet: diacylglycerol O-acyltransferase homolog 1 (mouse)
• Masse moléculaire calculée: 488 aa, 55 kDa
• Poids moléculaire observé: 50-57 kDa
• Numéro d’acquisition GenBank: BC015762
• Symbole du gène: DGAT1
• Identification du gène (NCBI): 8694
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu
		Nat Commun; O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.; Authors - Yunfan Yang
mouse	WB	J Cachexia Sarcopenia Muscle; Exogenous H2 S promotes ubiquitin-mediated degradation of SREBP1 to alleviate diabetic cardiomyopathy via SYVN1 S-sulfhydration; Authors - Shiwu Zhang
		Cell Mol Gastroenterol Hepatol; Lipid Storage and Interferon Response Determine the Phenotype of Ground Glass Hepatocytes in Mice and Humans.; Authors - Yuri Churin
human	IHC	Front Oncol; Identification of an Aging-Related Gene Signature in Predicting Prognosis and Indicating Tumor Immune Microenvironment in Breast Cancer.; Authors - Wenchang Lv
mouse	WB,IF	J Virol; Lipid droplets are beneficial for rabies virus replication by facilitating viral budding; Authors - Jianqing Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kelly (Verified Customer) (10-02-2024)

Great antibody

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: IMMUNOHISTOCHEMISTRY
• Cell Tissue Type: Antibody

FH

Kelly (Verified Customer) (09-21-2024)

Great antibody

• Applications: Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: IMMUNOFLUORESCENCE
• Cell Tissue Type: Antibody

FH

Kelly (Verified Customer) (09-06-2024)

Great antibody

• Applications: Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: IMMUNOPRECIPITATION
• Cell Tissue Type: Antibody

FH

Kelly (Verified Customer) (09-04-2024)

• Applications: Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: IMMUNOFLUORESCENCE
• Cell Tissue Type: Antibody

FH

Kel (Verified Customer) (07-04-2023)

Great product.

• Applications: Immunofluorescence, Protein purification

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