Anticorps Monoclonal anti-DGCR8

• Phare
• Validé par KD/KO

DGCR8 Monoclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4D11A3

N° de cat : 60084-1-PBS

Synonymes

C22orf12, DGCR8, DGCRK6, Gy1

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Galerie de données de validation

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  WB analysis using 60084-1-Ig (same clone as 60084-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60084-1-Ig (same clone as 60084-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 60084-1-Ig (same clone as 60084-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60084-1-Ig (same clone as 60084-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 60084-1-Ig (same clone as 60084-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 60084-1-Ig (same clone as 60084-1-PBS)

  IP result of anti-DGCR8 (IP:60084-1-Ig, 5ug; Detection:60084-1-Ig 1:500) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60084-1-PBS cible DGCR8 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DGCR8 Protéine recombinante Ag4871
• Nom complet: DiGeorge syndrome critical region gene 8
• Masse moléculaire calculée: 773 aa, 86 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC009323
• Symbole du gène: DGCR8
• Identification du gène (NCBI): 54487
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

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