Anticorps Polyclonal de lapin anti-DGKA

• Phare
• Validé par KD/KO

DGKA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 11547-1-AP

Synonymes

80 kDa diacylglycerol kinase, DAG kinase alpha, DAGK, DAGK1, DGK alpha, DGKA, Diacylglycerol kinase alpha, Diglyceride kinase alpha

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Galerie de données de validation

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  WB analysis using 11547-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11547-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 11547-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11547-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11547-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 11547-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11547-1-AP

  IP Result of anti-DGKA (IP:11547-1-AP, 3ug; Detection:11547-1-AP 1:1000) with Jurkat cells lysate 1500ug.

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  IHC staining of human gliomas using 11547-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11547-1-AP (DGKA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11547-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11547-1-AP (DGKA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 11547-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11547-1-AP (DGKA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 11547-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11547-1-AP (DGKA antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of Jurkat using 11547-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DGKA antibody (11547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Jurkat using 11547-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DGKA antibody (11547-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 11547-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DGKA (11547-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, cellules MOLT-4, tissu de cervelet de souris, tissu de cervelet humain
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Jurkat,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11547-1-AP cible DGKA dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DGKA Protéine recombinante Ag2075
• Nom complet: diacylglycerol kinase, alpha 80kDa
• Masse moléculaire calculée: 735 aa, 83 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC023523
• Symbole du gène: DGKA
• Identification du gène (NCBI): 1606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.

Publications

Species	Application	Title
		Nat Commun; Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.; Authors - David Novo
human	WB	Nat Chem Biol; Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering.; Authors - Timothy B Ware ; KD Validated
human	WB	Nat Commun; Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis.; Authors - Christoph Weigel
human	WB	Cancer Discov; Diacylglycerol Kinase α Is a Critical Signaling Node and Novel Therapeutic Target in Glioblastoma and Other Cancers.; Authors - Dominguez Charli L CL ; KD Validated
human	WB,IHC	Clin Cancer Res; DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 Signaling.; Authors - Jie Li ; KD Validated
mouse	WB	J Immunother Cancer; Diacylglycerol kinase ζ limits IL-2-dependent control of PD-1 expression in tumor-infiltrating T lymphocytes.; Authors - Javier Arranz-Nicolás