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Anticorps Polyclonal de lapin anti-DGKK-Specific

DGKK-Specific Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20090-1-PBS

Synonymes

Galerie de données de validation

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  • Western Blot (WB) analysis of human testis tissue using DGKK-Specific Polyclonal antibody (20090-1-AP)

    WB analysis of human testis using 20090-1-AP (same clone as 20090-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using DGKK-Specific Polyclonal antibody (20090-1-AP)

    WB analysis of human testis using 20090-1-AP (same clone as 20090-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using DGKK-Specific Polyclonal antibody (20090-1-AP)

    WB analysis of HuH-7 using 20090-1-AP (same clone as 20090-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DGKK-Specific Polyclonal antibody (20090-1-AP)

    IF Staining of HepG2 using 20090-1-AP (same clone as 20090-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using DGKK antibody 20090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

Informations sur le produit

20090-1-PBS cible DGKK-Specific dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet diacylglycerol kinase, kappa
Masse moléculaire calculée 142 kDa
Poids moléculaire observé 135-140 kDa
Numéro d’acquisition GenBankNM_001013742
Symbole du gène DGKK
Identification du gène (NCBI) 139189
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DGKK Belongs to the eukaryotic diacylglycerol kinase family. DGKK phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). DGKK catalyze the reaction: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. The antibody is specific to DGKK.

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