Anticorps Polyclonal de lapin anti-DHCR24

• Phare
• Validé par KD/KO

DHCR24 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(18)

N° de cat : 10471-1-AP

Synonymes

DHC24, 24-dehydrocholesterol reductase, 3-beta-hydroxysterol Delta-24-reductase, Delta(24)-sterol reductase, EC:1.3.1.72

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Galerie de données de validation

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  WB analysis using 10471-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10471-1-AP (DHCR24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10471-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10471-1-AP (DHCR24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10471-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10471-1-AP (DHCR24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse liver using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10471-1-AP (DHCR24 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules L02, cellules MCF-7, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du sein humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 2 publications below

Informations sur le produit

10471-1-AP cible DHCR24 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHCR24 Protéine recombinante Ag0756
• Nom complet: 24-dehydrocholesterol reductase
• Masse moléculaire calculée: 60 kDa, 516 aa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC004375
• Symbole du gène: DHCR24
• Identification du gène (NCBI): 1718
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The DHCR24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).

Publications

Species	Application	Title
mouse,human	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
mouse	WB	EMBO Mol Med; Shaping immune landscape of colorectal cancer by cholesterol metabolites; Authors - Yibing Bai
human	WB	Cell Death Differ; Efferocytosis potentiates the expression of arachidonate 15-lipoxygenase (ALOX15) in alternatively activated human macrophages through LXR activation.; Authors - Ryan G Snodgrass
human	WB	Commun Biol; Cholesterol mediated ferroptosis suppression reveals essential roles of Coenzyme Q and squalene; Authors - Qi Sun ; KD Validated
		J Neurosci; Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content.; Authors - Cortnie Hartwig
mouse	WB	NanoImpact; The alterations of miRNA and mRNA expression profile and their integration analysis induced by silica nanoparticles in spermatocyte cells.; Authors - Guiqing Zhou

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