Anticorps Polyclonal de lapin anti-DHFR

• Phare
• Validé par KD/KO

DHFR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15194-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15194-1-AP (same clone as 15194-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

  WB result of DHFR antibody (15194-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 15194-1-AP (same clone as 15194-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 15194-1-AP (same clone as 15194-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 15194-1-AP (same clone as 15194-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  IP experiment of Raji using 15194-1-AP (same clone as 15194-1-PBS)

  IP result of anti-DHFR (IP:15194-1-AP, 3ug; Detection:15194-1-AP 1:500) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

  Immunofluorescent analysis of HeLa cells using 15194-1-AP (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15194-1-PBS cible DHFR dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHFR Protéine recombinante Ag7331
• Nom complet: dihydrofolate reductase
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000192
• Symbole du gène: DHFR
• Identification du gène (NCBI): 1719
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).

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