Anticorps Recombinant de lapin anti-DHFR

• Phare
• Validé par KD/KO

DHFR Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

7P5

N° de cat : 81021-1-PBS

Synonymes

EC:1.5.1.3, DHFRP1, 7P5

---

Galerie de données de validation

• 

  WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 81021-1-RR (same clone as 81021-1-PBS)

  WB result of DHFR antibody (81021-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81021-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81021-1-RR against Human DHFR were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

81021-1-PBS cible DHFR dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DHFR Protéine recombinante Ag7331
• Nom complet: dihydrofolate reductase
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000192
• Symbole du gène: DHFR
• Identification du gène (NCBI): 1719
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).

{{ptg:RelatedPrimaryAntibodies}}