Anticorps Monoclonal anti-DHODH

DHODH Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2B2C6

N° de cat : 67977-1-Ig

Synonymes

DHOdehase, DHODH, dihydroorotate dehydrogenase, Dihydroorotate oxidase

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Galerie de données de validation

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  WB analysis of LNCaP using 67977-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67977-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67977-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67977-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67977-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67977-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67977-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules K-562, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

67977-1-Ig cible DHODH dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DHODH Protéine recombinante Ag6649
• Nom complet: dihydroorotate dehydrogenase
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC065245
• Symbole du gène: DHODH
• Identification du gène (NCBI): 1723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

Publications

Species	Application	Title
monkey	WB	Vet Res; Vidofludimus inhibits porcine reproductive and respiratory syndrome virus infection by targeting dihydroorotate dehydrogenase; Authors - Yuanqi Yang
mouse	WB	ACS Nano; Engineering Biodegradable Hollow Silica/Iron Composite Nanozymes for Breast Tumor Treatment through Activation of the "Ferroptosis Storm"; Authors - Panpan Xue
human,rat	WB	Front Pharmacol; Combination decoction of Astragalus mongholicus and Salvia miltiorrhiza mitigates pressure-overload cardiac dysfunction by inhibiting multiple ferroptosis pathways; Authors - Qiyao Xu

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