Anticorps Monoclonal anti-DHODH

DHODH Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B2C6

N° de cat : 67977-1-PBS

Synonymes

PYRD, EC:1.3.5.2, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate dehydrogenase

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Galerie de données de validation

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  WB analysis of LNCaP using 67977-1-Ig (same clone as 67977-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67977-1-Ig (same clone as 67977-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67977-1-Ig (same clone as 67977-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67977-1-Ig (same clone as 67977-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67977-1-Ig (same clone as 67977-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67977-1-Ig (same clone as 67977-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67977-1-Ig (same clone as 67977-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50416-1

  Cytometric bead array standard curve of MP50416-1, DHODH Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67977-2-PBS. Detection antibody: 67977-1-PBS. Standard:Ag6649. Range: 1.563-100 ng/mL.

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Informations sur le produit

67977-1-PBS cible DHODH dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DHODH Protéine recombinante Ag6649
• Nom complet: dihydroorotate dehydrogenase
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC065245
• Symbole du gène: DHODH
• Identification du gène (NCBI): 1723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

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