Anticorps Polyclonal de lapin anti-DHRS2

• Phare
• Validé par KD/KO

DHRS2 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15735-1-AP

Synonymes

DHRS2, Dicarbonyl reductase HEP27, HEP27, Protein D, SDR25C1

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Galerie de données de validation

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  WB analysis of SKOV-3 using 15735-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15735-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15735-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15735-1-AP

  IP Result of anti-DHRS2 (IP:15735-1-AP, 4ug; Detection:15735-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human liver cancer using 15735-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15735-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, tissu hépatique humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

15735-1-AP cible DHRS2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHRS2 Protéine recombinante Ag8508
• Nom complet: dehydrogenase/reductase (SDR family) member 2
• Masse moléculaire calculée: 300 aa, 32 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC007339
• Symbole du gène: DHRS2
• Identification du gène (NCBI): 10202
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.

Publications

Species	Application	Title
human	WB,IHC	Epigenetics; Decreased DHRS2 expression is associated with HDACi resistance and poor prognosis in ovarian cancer.; Authors - Yingyan Han ; KD Validated
human	WB	Oncol Rep; PMID: 31436301; Authors - Ji-Min Li ; KD Validated
human	WB	Cell Death Dis; REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.; Authors - Yunshang Chen
human	WB	Transl Oncol; STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression; Authors - Xiang Yang
human	IHC	Iran J Public Health; Auxiliary Diagnosis and Prognostic Value of Dehydrogenase/Reductase 2 (DHRS2) in Various Tumors; Authors - Zhongwu An