Anticorps Polyclonal de lapin anti-DHRS2

• Phare
• Validé par KD/KO

DHRS2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15735-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of SKOV-3 using 15735-1-AP (same clone as 15735-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

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  WB analysis using 15735-1-AP (same clone as 15735-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 15735-1-AP (same clone as 15735-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15735-1-AP (same clone as 15735-1-PBS)

  IP result of anti-DHRS2 (IP:15735-1-AP, 4ug; Detection:15735-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15735-1-AP (same clone as 15735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15735-1-AP (same clone as 15735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15735-1-PBS cible DHRS2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHRS2 Protéine recombinante Ag8508
• Nom complet: dehydrogenase/reductase (SDR family) member 2
• Masse moléculaire calculée: 300 aa, 32 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC007339
• Symbole du gène: DHRS2
• Identification du gène (NCBI): 10202
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.

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