Anticorps Recombinant de lapin anti-DHRS2

DHRS2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250977F7

N° de cat : 86309-2-RR

Synonymes

Dehydrogenase/reductase SDR family member 2, mitochondrial, Dicarbonyl reductase HEP27, EC:1.1.1.-, HEP27, Protein D

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Galerie de données de validation

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  WB analysis using 86309-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86309-2-RR (DHRS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 86309-2-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DHRS2 antibody (86309-2-RR, Clone: 250977F7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86309-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86309-2-RR against Human DHRS2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, cellules U2OS
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86309-2-RR cible DHRS2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DHRS2 Protéine recombinante Ag8508
• Nom complet: dehydrogenase/reductase (SDR family) member 2
• Masse moléculaire calculée: 300 aa, 32 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC007339
• Symbole du gène: DHRS2
• Identification du gène (NCBI): 10202
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.

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