Anticorps Polyclonal de lapin anti-DHRS3

• Phare
• Validé par KD/KO

DHRS3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 15393-1-AP

Synonymes

DD83.1, DHRS3, RDH17, retSDR1, Rsdr1, SDR1, SDR16C1

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Galerie de données de validation

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  WB analysis using 15393-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15393-1-AP (DHRS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15393-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15393-1-AP (DHRS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 15393-1-AP

  IP Result of anti-DHRS3 (IP:15393-1-AP, 4ug; Detection:15393-1-AP 1:500) with A375 cells lysate 3600ug.

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  IHC staining of mouse liver using 15393-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15393-1-AP (DHRS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15393-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15393-1-AP (DHRS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15393-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DHRS3 antibody (15393-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules A375
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

15393-1-AP cible DHRS3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, insecte
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHRS3 Protéine recombinante Ag7589
• Nom complet: dehydrogenase/reductase (SDR family) member 3
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC002730
• Symbole du gène: DHRS3
• Identification du gène (NCBI): 9249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHRS3(Short-chain dehydrogenase/reductase 3) is also named as retSDR1,DD83.1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH. It is detected high levels of expression in fetal kidney, liver, and lung and in adult heart, placenta, lung, liver, kidney, pancreas, thyroid, testis, stomach, trachea, spinal cord and lower levels in skeletal muscle, intestine, and lymph node. retSDR1 is barely detectable in adrenals, brain, thymus, and hematopoietic tissues(PMID:11861404). retSDR1 may play a more general role in retinol metabolism since its expression was observed in many fetal and adult tissues(PMID:20543567).

Publications

Species	Application	Title
human	WB	J Clin Endocrinol Metab; Elevated periimplantation uterine natural killer cell density in human endometrium is associated with impaired corticosteroid signaling in decidualizing stromal cells.; Authors - Kuroda Keiji K
mouse	IHC	FASEB J; The retinaldehyde reductase DHRS3 is essential for preventing the formation of excess retinoic acid during embryonic development.; Authors - Billings Sara E SE
human,mouse	WB	J Biol Chem; Retinol dehydrogenase 11 is essential for the maintenance of retinol homeostasis in liver and testis in mice.; Authors - Olga V Belyaeva ; KD Validated
mouse	WB	FASEB J; Liver regeneration and ethanol detoxification: A new link in YAP regulation of ALDH1A1 during alcohol-related hepatocyte damage.; Authors - Junmei Zhou
human	WB	Mol Endocrinol; Induction of 11β-HSD 1 and activation of distinct mineralocorticoid receptor- and glucocorticoid receptor-dependent gene networks in decidualizing human endometrial stromal cells.; Authors - Kuroda Keiji K
human	WB,IF	J Biol Chem; Retinol dehydrogenase 10 but not retinol/sterol dehydrogenase(s) regulates the expression of retinoic acid-responsive genes in human transgenic skin raft culture.; Authors - Lee Seung-Ah SA