Anticorps Polyclonal de lapin anti-DHRS4

DHRS4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15279-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of rat liver using 15279-1-AP (same clone as 15279-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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  WB analysis using 15279-1-AP (same clone as 15279-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 15279-1-AP (same clone as 15279-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15279-1-AP (same clone as 15279-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 15279-1-AP (same clone as 15279-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 15279-1-AP (same clone as 15279-1-PBS)

  IP result of anti-DHRS4 (IP:15279-1-AP, 4ug; Detection:15279-1-AP 1:400) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 15279-1-AP (same clone as 15279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 15279-1-AP (same clone as 15279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervix using 15279-1-AP (same clone as 15279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervix using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervix using 15279-1-AP (same clone as 15279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervix using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15279-1-PBS cible DHRS4 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHRS4 Protéine recombinante Ag7398
• Nom complet: dehydrogenase/reductase (SDR family) member 4
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC003019
• Symbole du gène: DHRS4
• Identification du gène (NCBI): 10901
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DHRS4(Dehydrogenase/reductase SDR family member 4) is also named as PHCR , NRDR ,PSCD , SCAD-SRL, and belongs to the short-chain dehydrogenases/reductases (SDR) family. It can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Human DHRS4 is a tetramer composed of 27 kDa subunits, which is inactivated at low temperature without dissociation into subunits(PMID:18571493).

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