Anticorps Polyclonal de lapin anti-DHRS9

DHRS9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14560-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14560-1-AP (same clone as 14560-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14560-1-AP (same clone as 14560-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse trachea using 14560-1-AP (same clone as 14560-1-PBS)

  mouse trachea tissue were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14560-1-AP (same clone as 14560-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14560-1-AP (DHRS9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14560-1-AP (same clone as 14560-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14560-1-AP (DHRS9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 14560-1-AP (same clone as 14560-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using DHRS9 antibody (14560-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 14560-1-AP (same clone as 14560-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 14560-1-AP (DHRS9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14560-1-PBS cible DHRS9 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHRS9 Protéine recombinante Ag6116
• Nom complet: dehydrogenase/reductase (SDR family) member 9
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC058883
• Symbole du gène: DHRS9
• Identification du gène (NCBI): 10170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Dehydrogenase/reductase 9(DHRS9), is a member of the short-chain dehydrogenases/reductases (SDR) family. DHRS9 has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. DHRS9 has 4 isoforms with molecular weight of 19, 30, 35, 42 kDa, and demonstrates oxidoreductase activity toward hydroxysteroids. DHRS9 is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus.

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