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Anticorps Recombinant de lapin anti-DHTKD1

DHTKD1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251575H1

N° de cat : 86661-3-RR

Synonymes

2-oxoadipate dehydrogenase complex component E1, 2-oxoadipate dehydrogenase, mitochondrial, Alpha-KADH-E1, Alpha-ketoadipate dehydrogenase, Dehydrogenase E1 and transketolase domain-containing protein 1

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using DHTKD1 Recombinant monoclonal antibody (86661-3-RR)

    WB analysis using 86661-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86661-3-RR (DHTKD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86661-3-RR

    Affinity and Kinetic Characterization of 86661-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86661-3-RR against Human DHTKD1 were performed. The affinity constant is 1.99 nM.

Applications testées

Résultats positifs en WBcellules L02, cellules HepG2, cellules MCF-7, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

86661-3-RR cible DHTKD1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène DHTKD1 Protéine recombinante Ag26515
Nom complet dehydrogenase E1 and transketolase domain containing 1
Masse moléculaire calculée 103 kDa
Poids moléculaire observé 103 kDa
Numéro d’acquisition GenBankBC007955
Symbole du gène DHTKD1
Identification du gène (NCBI) 55526
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHTKD1 (Dehydrogenase E1 And Transketolase Domain Containing 1), also named as 2-aminoadipic 2-oxoadipic aciduria protein, OADC-E1, OADH-E1, is a lesser-studied E1 enzyme among the family of 2-oxoacid dehydrogenases (PMID: 32695416). DHTKD1 was expressed ubiquitously in various tissues and was proposed to localize to the mitochondria (PMID: 23141294). DHTKD1 plays a critical role in energy production in mitochondria, suppression of DHTKD1 leads to impaired mitochondrial biogenesis and increased cell apoptosis (PMID: 24076469).

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