Anticorps Recombinant de lapin anti-DHX15

• Phare
• Validé par KD/KO

DHX15 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1F13

Avis (2)

N° de cat : 82137-1-RR

Synonymes

EC:3.6.4.13, DDX15, DBP1, ATP-dependent RNA helicase DHX15, ATP-dependent RNA helicase #46

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Galerie de données de validation

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  WB analysis of HeLa using 82137-1-RR

  WB result of DHX15 antibody (82137-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX15 transfected HeLa cells.

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  WB analysis using 82137-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82137-1-RR (DHX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 82137-1-RR

  IP result of anti-DHX15 (IP:82137-1-RR, 4ug; Detection:82137-1-RR 1:8000) with NIH/3T3 cells lysate 1320 ug.

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  IHC staining of mouse testis using 82137-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 82137-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82137-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 82137-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 82137-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:2500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HeLa using 82137-1-RR

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DHX15 (82137-1-RR, Clone:1F13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 82137-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82137-1-RR against Human DHX15 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules A431, cellules C6, cellules HeLa, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IP: cellules NIH/3T3,
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82137-1-RR cible DHX15 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DHX15 Protéine recombinante Ag2895
• Nom complet: DEAH (Asp-Glu-Ala-His) box polypeptide 15
• Masse moléculaire calculée: 795 aa, 91 kDa
• Poids moléculaire observé: 90-95 kDa
• Numéro d’acquisition GenBank: BC035974
• Symbole du gène: DHX15
• Identification du gène (NCBI): 1665
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kDa.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (12-16-2024)

Great antibody for Dhx15 detection! On the picture, red asterisk indicates the non-specific band that comes from the previous round of staining with another antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: RKO

DHX15 Antibody Western Blot validation (1:10000 dilution) in RKO (Cat no:82137-1-RR)

FH

Tsimafei (Verified Customer) (08-12-2024)

Excellent and sensitive antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: mESCs

DHX15 Antibody Western Blot validation (1:10000 dilution) in mESCs (Cat no:82137-1-RR)

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