Anticorps Polyclonal de lapin anti-DHX29

DHX29 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB,ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 13923-1-AP

Synonymes

DDX29, DEAH box protein 29, DHX29, Nucleic acid helicase DDXx

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Galerie de données de validation

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  WB analysis of HEK-293 using 13923-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13923-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13923-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 13923-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13923-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13923-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules HT-1080, cellules K-562, cellules MCF-7, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13923-1-AP cible DHX29 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHX29 Protéine recombinante Ag4938
• Nom complet: DEAH (Asp-Glu-Ala-His) box polypeptide 29
• Masse moléculaire calculée: 155 kDa
• Poids moléculaire observé: 150-155 kDa
• Numéro d’acquisition GenBank: BC056219
• Symbole du gène: DHX29
• Identification du gène (NCBI): 54505
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lilas (Verified Customer) (06-29-2021)

Used antibody at a 1:1000 dilution in 5% BSA, and overnight incubation at 4 degrees. A very sharp band detected at around 155 kDA using western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma cell lines