Anticorps Polyclonal de lapin anti-DHX32

• Phare
• Validé par KD/KO

DHX32 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 19808-1-AP

Synonymes

DDX32, DEAD/H box 32, DEAD/H helicase like protein 1, DEAH box protein 32, DHLP1, DHX32, HuDDX32

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Galerie de données de validation

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  WB analysis of mouse kidney using 19808-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 19808-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19808-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 19808-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19808-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19808-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 19808-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 19808-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules A431, cellules COLO 320, tissu de côlon de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

19808-1-AP cible DHX32 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHX32 Protéine recombinante Ag13834
• Nom complet: DEAH (Asp-Glu-Ala-His) box polypeptide 32
• Masse moléculaire calculée: 743 aa, 84 kDa
• Poids moléculaire observé: 84 kDa, 75 kDa
• Numéro d’acquisition GenBank: BC002473
• Symbole du gène: DHX32
• Identification du gène (NCBI): 55760
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHX32, a new member of the DEAH subfamily of DEAD box proteins, is a novel putative RNA helicase with an activation-dependent paten of expression and characterized by a conserved helicase domain consisting of 7 to 8 conserved motifs. RNA helicases of the DEAD box family are highly conserved enzymes that are required for RNA metabolism from transcription to degradation and are therefore important players in gene expression. DHX32 may play an important role in the development of colorectal cancer. This is a rabbit polyclonal anti-DHX32 antibody raised against part of the C-terminal chain of human DHX32.

Publications

Species	Application	Title
human	WB,IHC	Oncol Lett; DHX32 expression is an indicator of poor breast cancer prognosis.; Authors - Meng Wang
human	WB,IHC	Transl Cancer Res; Silencing of DHX32 increases the proliferation of liver cancer cells.; Authors - Min-Jing Cai ; KD Validated