Anticorps Monoclonal anti-DHX32

• Phare
• Validé par KD/KO

DHX32 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

Indirect ELISA, WB

Conjugaison

Non conjugué

CloneNo.

2A4G9

N° de cat : 68306-1-PBS

Synonymes

HuDDX32, EC:3.6.4.13, DHLP1, DEAD/H helicase-like protein 1, DDX32

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Galerie de données de validation

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  WB analysis using 68306-1-Ig (same clone as 68306-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68306-1-Ig (same clone as 68306-1-PBS)

  WB result of DHX32 antibody (68306-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX32 transfected A549 cells. This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68306-1-Ig (same clone as 68306-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 68306-1-Ig (same clone as 68306-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68306-1-Ig (same clone as 68306-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 68306-1-Ig (same clone as 68306-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug DHX32 Monoclonal antibody (68306-1-Ig, Clone:2A4G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68306-1-PBS cible DHX32 dans les applications de Indirect ELISA, WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DHX32 Protéine recombinante Ag13956
• Nom complet: DEAH (Asp-Glu-Ala-His) box polypeptide 32
• Masse moléculaire calculée: 743 aa, 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC002473
• Symbole du gène: DHX32
• Identification du gène (NCBI): 55760
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DHX32, an RNA helicase, belongs to the DEAH box RNA helicase family by virtue of its homology to the conserved helicase domain. DHX32 is abundantly expressed in human and murine models, participating in cell transcription and differentiation, and being capable of regulating T-cell apoptosis. DHX32 is also associated with some malignant tumors, such as lymphoma, colorectal cancer, and breast cancer (PMID: 35117530). DHX32 has 2 isoforms with molecular weights of 75 and 84 kDa, respectively.

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