Anticorps Polyclonal de lapin anti-DHX36

• Phare
• Validé par KD/KO

DHX36 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13159-1-PBS

Synonymes

G4R1, EC:3.6.4.12, DEAH-box protein 36, DEAD/H box polypeptide 36, DDX36

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Galerie de données de validation

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  WB analysis using 13159-1-AP (same clone as 13159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 13159-1-AP (same clone as 13159-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 13159-1-AP (same clone as 13159-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 13159-1-AP (same clone as 13159-1-PBS)

  IP result of anti-DHX36 (IP:13159-1-AP, 4ug; Detection:13159-1-AP 1:500) with A431 cells lysate 1000 ug. This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 13159-1-AP (same clone as 13159-1-PBS)

  IP result of anti-DHX36 (IP:13159-1-AP, 4ug; Detection:13159-1-AP 1:500) with PC-3 cells lysate 1760 ug. This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13159-1-AP (same clone as 13159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13159-1-AP (same clone as 13159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13159-1-AP (same clone as 13159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13159-1-AP (same clone as 13159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13159-1-PBS cible DHX36 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHX36 Protéine recombinante Ag3799
• Nom complet: DEAH (Asp-Glu-Ala-His) box polypeptide 36
• Masse moléculaire calculée: 1008 aa, 115 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC036035
• Symbole du gène: DHX36
• Identification du gène (NCBI): 170506
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DHX36, also known as RNA associated with AU-rich element (RHAU) and G4R1, is an adenosine triphosphate (ATP)-dependent RNA helicase that belongs to the DExH/D family of RNA modifying enzymes. Proteins in this family involve in a wide range of cellular functions including RNA splicing, ribosome assembly, messenger RNA (mRNA) stability, microRNA processing, ribonucleoprotein remodeling and RNA trafficking. DHX36 is an RNA helicase that has specificity for DNA and RNA G4-quadruplexes, and resolves G4 structures into separate nucleotide strands for metabolic processing of G-rich sequence.

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