Anticorps Polyclonal de lapin anti-DHX58/LGP2

• Phare
• Validé par KD/KO

DHX58/LGP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 11355-1-AP

Synonymes

D11LGP2, D11lgp2e, DHX58, DHX58/LGP2, LGP2, Protein D11Lgp2 homolog, RIG I like receptor LGP2, RLR

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Galerie de données de validation

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  WB analysis of HEK-293 using 11355-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11355-1-AP (DHX58/LGP2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11355-1-AP

  IP Result of anti-DHX58/LGP2 (IP:11355-1-AP, 3ug; Detection:11355-1-AP 1:300) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human breast cancer using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human nephroblastoma using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human nephroblastoma using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:10 (under 10x lens).

• 

  IHC staining of human breast cancer using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu hépatique de rat, tissu rénal de rat
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de néphroblastome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

11355-1-AP cible DHX58/LGP2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHX58/LGP2 Protéine recombinante Ag1910
• Nom complet: DEXH (Asp-Glu-X-His) box polypeptide 58
• Masse moléculaire calculée: 678 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC014949
• Symbole du gène: DHX58
• Identification du gène (NCBI): 79132
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHX58(Probable ATP-dependent RNA helicase DHX58) is also named as D11LGP2E, LGP2 and belongs to the RLR subfamily. DHX58, originally identified as a highly expressed gene in mammary tumors, is another cytoplasmic DEX(D/H)-box helicase that can recognize RNA(PMID: 18411269). It acts as a positive, but not negative, regulator of RIG-I-and MDA5-dependent recognition of RNA virus infection and plays a pivotal role in antiviral responses in vivo(PMID:20080593).

Publications

Species	Application	Title
mouse	WB	Nat Commun; RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection.; Authors - Amy E L Stone ; KO Validated
human	WB	Nucleic Acids Res; miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular pyrophosphatases, DOM3Z and DUSP11.; Authors - Yalena Amador-Cañizares
human	WB	EMBO Rep; RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling.; Authors - Jean-Patrick Parisien
human,pig,monkey	WB,IF,IP	J Virol; LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling; Authors - Zhenbang Zhu ; KD Validated
human,mouse	WB	J Virol; Ambivalent role of the innate immune response in rabies virus pathogenesis.; Authors - Chopy Damien D
human,mouse	WB,IHC,IP	Cancer Sci; Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.; Authors - Li-Ling Lin