Anticorps Monoclonal anti-DHX9

• Phare
• Validé par KD/KO

DHX9 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

1B12C10

N° de cat : 67153-1-Ig

Synonymes

1B12C10, ATP-dependent RNA helicase A, DDX9, DExH-box helicase 9, EC:3.6.4.13

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Galerie de données de validation

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  WB analysis using 67153-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of HEK-293 using 67153-1-Ig

  WB result of DHX9 antibody (67153-1-Ig; 1:400000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX9 transfected HEK-293 cells.

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  WB analysis using 67153-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67153-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67153-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67153-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 67153-1-Ig

  IP result of anti-DHX9 (IP:67153-1-Ig, 5ug; Detection:67153-1-Ig 1:20000) with HeLa cells lysate 2000 ug.

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  IHC staining of mouse brain using 67153-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67153-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67153-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67153-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67153-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67153-1-Ig (DHX9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules THP-1
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

67153-1-Ig cible DHX9 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DHX9 Protéine recombinante Ag12104
• Nom complet: DEAH (Asp-Glu-Ala-His) box polypeptide 9
• Masse moléculaire calculée: 1270 aa, 141 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC014246
• Symbole du gène: DHX9
• Identification du gène (NCBI): 1660
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].

Publications

Species	Application	Title
mouse,human	WB,IF	Sci Adv; Nucleic DHX9 cooperates with STAT1 to transcribe interferon-stimulated genes; Authors - Xingxing Ren
human	WB	Front Oncol; MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing; Authors - Tamara Vital
mouse	WB,RIP,IP,IF,CoIP	Cell Rep; Virus-induced lncRNA-BTX allows viral replication by regulating intracellular translocation of DHX9 and ILF3 to induce innate escape; Authors - Yang Cao
human	IF	Nat Commun; DHX9 maintains epithelial homeostasis by restraining R-loop-mediated genomic instability in intestinal stem cells; Authors - Xingxing Ren
human	WB	J Am Chem Soc; Polymerase η Recruits DHX9 Helicase to Promote Replication across Guanine Quadruplex Structures; Authors - Feng Tang
human		Nat Commun; Virus-modified paraspeckle-like condensates are hubs for viral RNA processing and their formation drives genomic instability; Authors - Katherine L Harper

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