Anticorps Polyclonal de lapin anti-DIAPH3

• Phare
• Validé par KD/KO

DIAPH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14342-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

  WB result of DIAPH3 antibody (14342-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 14342-1-AP (same clone as 14342-1-PBS)

  WB result of DIAPH3 antibody (14342-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HEK-293 cells. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 14342-1-AP (same clone as 14342-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 14342-1-AP (same clone as 14342-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

  IP result of anti-DIAPH3 (IP:14342-1-AP, 4ug; Detection:14342-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DIAPH3 antibody (14342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

  Immunofluorescent analysis of HeLa cells using 14342-1-AP (DIAPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14342-1-PBS cible DIAPH3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DIAPH3 Protéine recombinante Ag5656
• Nom complet: diaphanous homolog 3 (Drosophila)
• Masse moléculaire calculée: 137 kDa
• Poids moléculaire observé: 125-137 kDa
• Numéro d’acquisition GenBank: BC048963
• Symbole du gène: DIAPH3
• Identification du gène (NCBI): 81624
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

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