Anticorps Recombinant de lapin anti-DIAPH3

• Phare
• Validé par KD/KO

DIAPH3 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242950G7

N° de cat : 85701-1-RR

Synonymes

DIAP3, Diaphanous-related formin-3, DRF3, MDia2

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Galerie de données de validation

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  WB analysis of HeLa using 85701-1-RR

  WB result of DIAPH3 antibody (85701-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells.

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  WB analysis using 85701-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85701-1-RR (DIAPH3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 85701-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using DIAPH3 antibody (85701-1-RR, Clone: 242950G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85701-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85701-1-RR against Human DIAPH3 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules DU 145, cellules HEK-293, cellules MCF-7, cellules U2OS
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85701-1-RR cible DIAPH3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DIAPH3 Protéine recombinante Ag6631
• Nom complet: diaphanous homolog 3 (Drosophila)
• Masse moléculaire calculée: 137 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC048963
• Symbole du gène: DIAPH3
• Identification du gène (NCBI): 81624
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

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