Anticorps Monoclonal anti-DICER1

DICER1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1C8E2

Avis (3)

N° de cat : 68375-1-Ig

Synonymes

DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928

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Galerie de données de validation

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  WB analysis of U2OS using 68375-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68375-1-Ig (DICER1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 68375-1-Ig

  IP result of anti-DICER1 (IP:68375-1-Ig, 4ug; Detection:68375-1-Ig 1:500) with Jurkat cells lysate 1560 ug.

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  IF Staining of HepG2 using 68375-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DICER1 antibody (68375-1-Ig, Clone: 1C8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP, cellules Ramos, cellules Sp2/0
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68375-1-Ig cible DICER1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DICER1 Protéine recombinante Ag30368
• Nom complet: dicer 1, ribonuclease type III
• Masse moléculaire calculée: 219 kDa
• Poids moléculaire observé: 240-250 kDa
• Numéro d’acquisition GenBank: NM_030621
• Symbole du gène: DICER1
• Identification du gène (NCBI): 23405
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DICER1 functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. DICER1 cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, and producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (11-10-2024)

Great antibody, that were used to probe for Dicer1 in human RKO cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

DICER1 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:68375-1-Ig)

FH

Tsimafei (Verified Customer) (08-07-2024)

Excellent antibody for Dicer1

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

DICER1 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:68375-1-Ig)

FH

Laurens (Verified Customer) (08-01-2023)

Antibody worked excellently on western blot. On par or even better than the D38E7 antibody from Cell Signalling, despite using a lower dilution of the Proteintech antibody. Will turn out to be a cheaper and better alternative.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293

DICER1 Antibody Western Blot validation (1:2000 dilution) in HEK293 (Cat no:68375-1-Ig)

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